Hotspot

ndhE-psaC

SSC · 124208-124667 · 460 bp

Back to Brahea

Pi 0.271
MarkerSeek score 70.30
Primer available yes
Label ndhE-psaC

Feature Metadata

label_name
ndhE-psaC
feature_type
igs
region
SSC
length_bp
460
variable_sites
220
pi
0.271
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-psaC_p1 CTGAAGCAGCTATTGGGCTA ATCGAGTACGGGCTTTTGTC 50.0 50.0 57.4 58.0 805-1123 bp 0.5 59.3
ndhE-psaC_p2 TGAAGCAGCTATTGGGCTAG ATCGAGTACGGGCTTTTGTC 50.0 50.0 57.4 58.0 804-1122 bp 0.5 59.3
ndhE-psaC_p3 TGTTATAGCTGTTGCGGCTG ATCGAGTACGGGCTTTTGTC 50.0 50.0 58.6 58.0 825-1143 bp 0.5 59.2
ndhE-psaC_p4 CTGAAGCAGCTATTGGGCTA ACATCCGTCCCAAGGTATCA 50.0 50.0 57.4 58.1 596-914 bp 0.5 58.4
ndhE-psaC_p5 TGAAGCAGCTATTGGGCTAG ACATCCGTCCCAAGGTATCA 50.0 50.0 57.4 58.1 595-913 bp 0.5 58.4