Hotspot

rpl32-trnL

Genome · 105767-106467 · 701 bp

Back to Brachypodium

Pi 0.0394

MarkerSeek score 70.50

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: Genome
length_bp: 701
variable_sites: 41
pi: 0.0394
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	CGACAACGAGCATCCAAAAC	TGTCCTGAAGAAACCCAACG	50.0	50.0	58.0	58.0	1119-1127 bp	1.0	83.2
rpl32-trnL_p2	CGACAACGAGCATCCAAAAC	TACAGAGCCCAGGAAAGGAA	50.0	50.0	58.0	58.0	1173-1181 bp	1.0	83.1
rpl32-trnL_p3	CGACAACGAGCATCCAAAAC	TTCGAACCGAGATGCTTCAG	50.0	50.0	58.0	58.0	834 bp	0.333333	52.9
rpl32-trnL_p4	CGACAACGAGCATCCAAAAC	AGAGCCCAGGAAAGGAAGAT	50.0	50.0	58.0	58.0	1170 bp	0.333333	52.8
rpl32-trnL_p5	TGGGGGATAGACTTCCGTAC	TTCGAACCGAGATGCTTCAG	55.0	50.0	57.9	58.0	1310 bp	0.333333	52.7