Hotspot

ndhF-rpl32

Genome · 104697-105574 · 878 bp

Back to Brachypodium

Pi 0.0263

MarkerSeek score 65.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 878
variable_sites: 33
pi: 0.0263
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCCACAAGTTCCC	ACCCTTTGGTTTTGGATGCT	50.0	45.0	58.0	57.9	1466-1502 bp	1.0	83.9
ndhF-rpl32_p2	CACATTCCCACAAGTTCCCA	ACCCTTTGGTTTTGGATGCT	50.0	45.0	58.0	57.9	1465-1501 bp	1.0	83.8
ndhF-rpl32_p3	ACACATTCCCACAAGTTCCC	GTTTTGGATGCTCGTTGTCG	50.0	50.0	58.0	58.0	1494 bp	0.333333	52.9
ndhF-rpl32_p4	CACATTCCCACAAGTTCCCA	GTTTTGGATGCTCGTTGTCG	50.0	50.0	58.0	58.0	1493 bp	0.333333	52.9
ndhF-rpl32_p5	GAAGAGGGAATTGTGCGGAT	GTTTTGGATGCTCGTTGTCG	50.0	50.0	58.0	58.0	1762 bp	0.333333	52.6