Hotspot

rpl23-psaI

Genome · 56565-57639 · 1075 bp

Back to Brachypodium

Pi 0.0164

MarkerSeek score 57.80

Primer available yes

Label rpl23-psaI

Feature Metadata

label_name: rpl23-psaI
feature_type: igs
region: Genome
length_bp: 1075
variable_sites: 26
pi: 0.0164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-psaI_p1	GCCTGTGTACAAGCTCGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1826-1854 bp	1.0	82.8
rpl23-psaI_p2	GCATTGGGTCGAACTCTTCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1285-1308 bp	1.0	82.8
rpl23-psaI_p3	GCCTGTGTACAAGCTCGTAA	ACTAAATCCAGCAGCAGTCG	50.0	50.0	57.9	58.0	2167-2195 bp	1.0	82.5
rpl23-psaI_p4	AGCATTGGGTCGAACTCTTC	ACTAAATCCAGCAGCAGTCG	50.0	50.0	57.9	58.0	1627-1650 bp	1.0	82.4
rpl23-psaI_p5	GCATTGGGTCGAACTCTTCT	ACTAAATCCAGCAGCAGTCG	50.0	50.0	57.9	58.0	1626-1649 bp	1.0	82.4