Hotspot

atpI-atpH

Genome · 31722-32101 · 380 bp

Back to Brachypodium

Pi 0.014

MarkerSeek score 63.10

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 380
variable_sites: 8
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	45.0	50.0	57.9	58.1	470-477 bp	1.0	80.1
atpI-atpH_p2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	45.0	50.0	57.9	58.1	473-480 bp	1.0	80.1
atpI-atpH_p3	TTTTGCAACATTAGCCGCAG	GCCAATCCAGCAGCAATAAC	45.0	50.0	57.9	57.8	482-489 bp	1.0	79.1
atpI-atpH_p4	CAACATTAGCCGCAGCCTAT	GCAATAACAGAAGCAGCAGC	50.0	50.0	58.4	58.1	465-472 bp	1.0	77.4
atpI-atpH_p5	CAACATTAGCCGCAGCCTAT	GCAGCAATAACAGAAGCAGC	50.0	50.0	58.4	58.1	468-475 bp	1.0	77.4