Hotspot

psbM-petN

Genome · 16639-17483 · 845 bp

Back to Brachypodium

Pi 0.0161

MarkerSeek score 66.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 845
variable_sites: 18
pi: 0.0161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCGCATTTATCGCTACTGCA	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	57.8	58.0	935-1010 bp	1.0	83.7
psbM-petN_p2	CGCATTTATCGCTACTGCAC	TCTTGCTTGGGCTGCTTTAA	50.0	45.0	57.7	58.0	934-1009 bp	1.0	83.1
psbM-petN_p3	TCGCATTTATCGCTACTGCA	CTTTCACTAGTGGTGTGGGG	45.0	55.0	57.8	57.8	895-970 bp	1.0	82.3
psbM-petN_p4	TCGCATTTATCGCTACTGCA	AGTAAGTCTTGCTTGGGCTG	45.0	50.0	57.8	57.8	941-1016 bp	1.0	82.2
psbM-petN_p5	TCGCTACTGCACTGTTCATT	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	57.5	58.0	926-1001 bp	1.0	81.8