Hotspot

trnD-psbM

Genome · 15738-16533 · 796 bp

Back to Brachypodium

Pi 0.0127

MarkerSeek score 63.40

Primer available yes

Label trnD-psbM

Feature Metadata

label_name: trnD-psbM
feature_type: igs
region: Genome
length_bp: 796
variable_sites: 15
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-psbM_p1	TTCAATTGGTCAGAGCACCG	TGCAGTAGCGATAAATGCGA	50.0	45.0	58.5	57.8	888-896 bp	1.0	76.7
trnD-psbM_p2	TTCAATTGGTCAGAGCACCG	GTGCAGTAGCGATAAATGCG	50.0	50.0	58.5	57.7	889-897 bp	1.0	76.1
trnD-psbM_p3	TAGGTATGCCATACACCCCG	TGCAGTAGCGATAAATGCGA	55.0	45.0	58.7	57.8	920-928 bp	1.0	75.2
trnD-psbM_p4	TTCAATTGGTCAGAGCACCG	AATGAACAGTGCAGTAGCGA	50.0	45.0	58.5	57.5	897-905 bp	1.0	74.8
trnD-psbM_p5	TAGGTATGCCATACACCCCG	GTGCAGTAGCGATAAATGCG	55.0	50.0	58.7	57.7	921-929 bp	1.0	74.6