Hotspot

trnG-trnI

Genome · 13455-13995 · 541 bp

Back to Brachypodium

Pi 0.024

MarkerSeek score 61.00

Primer available yes

Label trnG-trnI

Feature Metadata

label_name: trnG-trnI
feature_type: igs
region: Genome
length_bp: 541
variable_sites: 19
pi: 0.024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnI_p1	CATCGTTAGCTTGGAAGGCT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1793-1826 bp	1.0	83.1
trnG-trnI_p2	CGTTAGCTTGGAAGGCTAGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1790-1823 bp	1.0	82.9
trnG-trnI_p3	CCGAGCTGGAACAATATGCT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1503-1521 bp	1.0	82.7
trnG-trnI_p4	CATCGTTAGCTTGGAAGGCT	CCGATGACTTACGCCTTACC	50.0	55.0	58.0	58.2	1790-1823 bp	1.0	81.9
trnG-trnI_p5	CTTCGCGACTCTGTACTCAG	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1330 bp	0.333333	52.0