Hotspot

trnG-trnfM

Genome · 12096-12529 · 434 bp

Back to Brachypodium

Pi 0.02

MarkerSeek score 61.60

Primer available yes

Label trnG-trnfM

Feature Metadata

label_name: trnG-trnfM
feature_type: igs
region: Genome
length_bp: 434
variable_sites: 13
pi: 0.02
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnfM_p1	GTCGACCCAAGCGGATATAC	CGAGGCTCATAACCTTGAGG	55.0	55.0	57.9	57.8	590-592 bp	1.0	79.7
trnG-trnfM_p2	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	550-552 bp	1.0	79.7
trnG-trnfM_p3	GTCGACCCAAGCGGATATAC	CAGTTTGGTAGCTCACGAGG	55.0	55.0	57.9	58.3	605-607 bp	1.0	79.2
trnG-trnfM_p4	GCGAGCGTAGTTCAATGGTA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	565-567 bp	1.0	79.2
trnG-trnfM_p5	CGTAGCGAGCGTAGTTCAAT	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.2	57.8	554-556 bp	1.0	78.9