Hotspot

rps11

LSC · 77750-78166 · 417 bp

Back to Brachycorythis

Pi 0
MarkerSeek score 23.70
Primer available yes
Label rps11

Feature Metadata

label_name
rps11
feature_type
gene
region
LSC
length_bp
417
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
23.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11_p1 TTGAATCATTTCTTTCTCTTGAA TGGATATCCCCATATCTTTCT 26.1 38.1 52.6 52.8 511 bp 1.0 40.0
rps11_p2 ATTGAATCATTTCTTTCTCTTGA TGGATATCCCCATATCTTTCT 26.1 38.1 52.3 52.8 512 bp 1.0 40.0
rps11_p3 TGAATCATTTCTTTCTCTTGAAT TGGATATCCCCATATCTTTCT 26.1 38.1 52.3 52.8 510 bp 1.0 40.0
rps11_p4 TTGAATCATTTCTTTCTCTTGAA ATGGATATCCCCATATCTTTCT 26.1 36.4 52.6 53.5 512 bp 1.0 40.0
rps11_p5 TGAATCATTTCTTTCTCTTGAATT TGGATATCCCCATATCTTTCT 25.0 38.1 53.2 52.8 510 bp 1.0 40.0