Hotspot

rpl14

LSC · 79541-79909 · 369 bp

Back to Brachycorythis

Pi 0
MarkerSeek score 32.90
Primer available yes
Label rpl14

Feature Metadata

label_name
rpl14
feature_type
gene
region
LSC
length_bp
369
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14_p1 AGACACAATCCATTCATCGGA ACGCGGGTTTCTTTATCTCT 42.9 45.0 57.1 56.9 558 bp 1.0 56.4
rpl14_p2 AGACACAATCCATTCATCGGA GCGGGTTTCTTTATCTCTGGA 42.9 47.6 57.1 57.7 556 bp 1.0 54.6
rpl14_p3 AGACACAATCCATTCATCGGA AACGCGGGTTTCTTTATCTCT 42.9 42.9 57.1 57.7 559 bp 1.0 54.3
rpl14_p4 GAGACACAATCCATTCATCGGA ACGCGGGTTTCTTTATCTCT 45.5 45.0 58.2 56.9 559 bp 1.0 54.1
rpl14_p5 GAGACACAATCCATTCATCGG ACGCGGGTTTCTTTATCTCT 47.6 45.0 56.8 56.9 559 bp 1.0 53.9