Hotspot

rps3-rpl22

LSC · 82410-82487 · 78 bp

Back to Brachycorythis

Pi 0.0536

MarkerSeek score 78.10

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 78
variable_sites: 3
pi: 0.0536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	ATCCTTTGGTTTTGCGAACC	TGAAAAAGTTCAGACCCCGG	45.0	50.0	57.2	58.0	238-260 bp	1.0	78.3
rps3-rpl22_p2	CCAAGTCTGAAACCAAGTGGA	TGAAAAAGTTCAGACCCCGG	47.6	50.0	58.1	58.0	192-214 bp	1.0	76.4
rps3-rpl22_p3	TCCAAGTCTGAAACCAAGTGG	TGAAAAAGTTCAGACCCCGG	47.6	50.0	58.1	58.0	193-215 bp	1.0	76.4
rps3-rpl22_p4	AATCCTTTGGTTTTGCGAACC	TGAAAAAGTTCAGACCCCGG	42.9	50.0	57.9	58.0	239-261 bp	1.0	75.9
rps3-rpl22_p5	CCAAGTCTGAAACCAAGTGG	TGAAAAAGTTCAGACCCCGG	50.0	50.0	56.5	58.0	192-214 bp	1.0	73.4