Hotspot

rpl16-rps3

LSC · 81603-81761 · 159 bp

Back to Brachycorythis

Pi 0.0252

MarkerSeek score 67.00

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 4
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CACGAGTCACACACTAAGCA	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	57.9	58.0	300-302 bp	1.0	79.7
rpl16-rps3_p2	TCACGAGTCACACACTAAGC	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	57.6	58.0	301-303 bp	1.0	77.4
rpl16-rps3_p3	CACGAGTCACACACTAAGCA	CCCTTACAAACAATTCGCGC	50.0	50.0	57.9	58.4	285-287 bp	1.0	77.0
rpl16-rps3_p4	CACGAGTCACACACTAAGCA	CCTTACAAACAATTCGCGCT	50.0	45.0	57.9	57.4	284-286 bp	1.0	75.2
rpl16-rps3_p5	TCACGAGTCACACACTAAGC	CCCTTACAAACAATTCGCGC	50.0	50.0	57.6	58.4	286-288 bp	1.0	74.7