Hotspot

rpl36-infA

LSC · 78433-78558 · 126 bp

Back to Brachycorythis

Pi 0.0364

MarkerSeek score 75.50

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 4
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CCTACGAATCAGGCGACATT	TGATTCAACCAGGGGACGTA	50.0	50.0	57.8	58.4	225-241 bp	1.0	80.7
rpl36-infA_p2	CCTACGAATCAGGCGACATT	CCGGCGCAGTTTTATACGTA	50.0	50.0	57.8	58.4	285-301 bp	1.0	79.8
rpl36-infA_p3	CCTACGAATCAGGCGACATT	ATCCGGCGCAGTTTTATACG	50.0	50.0	57.8	58.8	287-303 bp	1.0	77.0
rpl36-infA_p4	CCTACGAATCAGGCGACATT	TATGATTCAACCAGGGGACG	50.0	50.0	57.8	57.0	227-243 bp	1.0	75.6
rpl36-infA_p5	CCTACGAATCAGGCGACATT	GATTCAACCAGGGGACGTAT	50.0	50.0	57.8	57.0	224-240 bp	1.0	75.6