Hotspot

trnS-GCU-trnG-GCC

LSC · 7941-8557 · 617 bp

Back to Brachycorythis

Pi 0.0211
MarkerSeek score 66.20
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
617
variable_sites
13
pi
0.0211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA AACGGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 724-733 bp 1.0 68.4
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC AACGGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 723-732 bp 1.0 68.4
trnS-GCU-trnG-GCC_p3 GATCAGGGGAATCGGAGAGA AACGGAAAGACCCCTTAACT 55.0 45.0 58.0 56.4 789-798 bp 1.0 68.3
trnS-GCU-trnG-GCC_p4 ATCAGGGGAATCGGAGAGAG AACGGAAAGACCCCTTAACT 55.0 45.0 58.0 56.4 788-797 bp 1.0 68.3
trnS-GCU-trnG-GCC_p5 CAAGTGATCAGGGGAATCGG AACGGAAAGACCCCTTAACT 55.0 45.0 58.0 56.4 794-803 bp 1.0 68.0