Hotspot

matK-trnK-UUU

LSC · 3236-4307 · 1072 bp

Back to Brachycorythis

Pi 0.00468

MarkerSeek score 61.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 5
pi: 0.00468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AAGGAGCAGCGGATATAGGA	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.7	57.9	1200-1207 bp	1.0	77.2
matK-trnK-UUU_p2	AGGAGCAGCGGATATAGGAA	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.7	57.9	1199-1206 bp	1.0	77.2
matK-trnK-UUU_p3	AAGGAGCAGCGGATATAGGA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	1182-1189 bp	1.0	76.7
matK-trnK-UUU_p4	AGGAGCAGCGGATATAGGAA	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.7	58.2	1182-1189 bp	1.0	76.7
matK-trnK-UUU_p5	AGGAGCAGCGGATATAGGAA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	1181-1188 bp	1.0	76.7