Hotspot

rpl16

LSC · 76476-77957 · 1482 bp

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Pi 0.0575

MarkerSeek score 75.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1482
variable_sites: 84
pi: 0.0575
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCAATTCCTCGGCAATCG	AAATAGCAGGCCGTCTAGGA	50.0	50.0	58.1	58.2	2224-2330 bp	1.0	82.2
rpl16_p2	GCTCCACTGTTATCCGCTAC	AAATAGCAGGCCGTCTAGGA	55.0	50.0	58.1	58.2	1947-2053 bp	1.0	82.1
rpl16_p3	AGCTCCACTGTTATCCGCTA	AAATAGCAGGCCGTCTAGGA	50.0	50.0	58.2	58.2	1948-2054 bp	1.0	81.5
rpl16_p4	AGCAGCTCCTATGACTCGAA	AAATAGCAGGCCGTCTAGGA	50.0	50.0	58.2	58.2	1984-2090 bp	1.0	81.3
rpl16_p5	TCTCAATTCCTCGGCAATCG	GGGTAGACTTCCCCTCCAAA	50.0	55.0	58.1	58.3	2169 bp	0.5	56.8