Hotspot

ccsA-ndhD

SSC · 105189-105375 · 187 bp

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Pi 0.0538

MarkerSeek score 75.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 187
variable_sites: 10
pi: 0.0538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCATTTGTAGCTTCGATAGGA	CTCTCGCTATCCGTTGACAG	42.9	55.0	55.9	57.9	355-365 bp	1.0	56.4
ccsA-ndhD_p2	GCATTTGTAGCTTCGATAGGA	TTGACAGGGTAGAGGCTCTC	42.9	55.0	55.9	58.1	342-352 bp	1.0	56.3
ccsA-ndhD_p3	GCATTTGTAGCTTCGATAGGA	GACAGGGTAGAGGCTCTCTT	42.9	55.0	55.9	57.8	340-350 bp	1.0	56.2
ccsA-ndhD_p4	GCATTTGTAGCTTCGATAGGA	TCTCTCGCTATCCGTTGACA	42.9	50.0	55.9	58.3	356-366 bp	1.0	55.3
ccsA-ndhD_p5	GCATTTGTAGCTTCGATAGGA	GTTGACAGGGTAGAGGCTCT	42.9	55.0	55.9	58.4	343-353 bp	1.0	53.9