Hotspot

rpl22-rps19

IRb · 79278-79350 · 73 bp

Back to Bouteloua

Pi 0.0411

MarkerSeek score 76.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 73
variable_sites: 3
pi: 0.0411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAGCCCGTTCTTCTTCTT	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.6	58.0	214 bp	1.0	78.1
rpl22-rps19_p2	CGTAGCCCGTTCTTCTTCTT	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.6	58.0	215 bp	1.0	78.1
rpl22-rps19_p3	TTCTTGCGAGTTTCCGTAGC	CAAATTGGGGGAATTCGTGC	50.0	50.0	58.6	58.0	228 bp	1.0	76.4
rpl22-rps19_p4	TTCTTGCGAGTTTCCGTAGC	GCAAATTGGGGGAATTCGTG	50.0	50.0	58.6	58.0	229 bp	1.0	76.4
rpl22-rps19_p5	CGTAGCCCGTTCTTCTTCTT	ATGGTAGGTCGCAAATTGGG	50.0	50.0	57.6	58.2	225 bp	1.0	76.2