Hotspot

clpP-psbB

LSC · 67107-67643 · 537 bp

Back to Bouteloua

Pi 0.0523

MarkerSeek score 75.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 27
pi: 0.0523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ATTCGTGATCTCGCAACGAA	TGCACCAAAGCCAAAACAAG	45.0	45.0	58.0	58.0	1137-1139 bp	1.0	83.6
clpP-psbB_p2	TTCGTGATCTCGCAACGAAT	TGCACCAAAGCCAAAACAAG	45.0	45.0	58.0	58.0	1136-1138 bp	1.0	83.6
clpP-psbB_p3	GGCTTCGTCTGCTGACATAA	TGCACCAAAGCCAAAACAAG	50.0	45.0	58.0	58.0	1554-1556 bp	1.0	83.6
clpP-psbB_p4	AATAAGCACTAGCCGGTTGG	TGCACCAAAGCCAAAACAAG	50.0	45.0	58.0	58.0	1399-1401 bp	1.0	83.1
clpP-psbB_p5	TCTAGCGTGAGGGAATGCTA	TGCACCAAAGCCAAAACAAG	50.0	45.0	57.9	58.0	1368-1370 bp	1.0	82.9