Hotspot

trnR-UCU-trnfM-CAU

LSC · 35929-36090 · 162 bp

Back to Bouteloua

Pi 0.0683

MarkerSeek score 76.40

Primer available yes

Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name: trnR-UCU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 11
pi: 0.0683
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnfM-CAU_p1	AGAAGACCTCTGTCCTATCCA	GTAGAGCTGCGTGGTAACTC	47.6	55.0	56.9	58.1	252-253 bp	1.0	66.7
trnR-UCU-trnfM-CAU_p2	AGAAGACCTCTGTCCTATCCA	TTCCCCGCTAGAAGGTAGAG	47.6	55.0	56.9	57.9	266-267 bp	1.0	66.6
trnR-UCU-trnfM-CAU_p3	AGGTTTAGAAGACCTCTGTCCT	TTCCCCGCTAGAAGGTAGAG	45.5	55.0	57.9	57.9	272-273 bp	1.0	65.9
trnR-UCU-trnfM-CAU_p4	AGGTTTAGAAGACCTCTGTCCT	GTAGAGCTGCGTGGTAACTC	45.5	55.0	57.9	58.1	258-259 bp	1.0	65.9
trnR-UCU-trnfM-CAU_p5	AGAAGACCTCTGTCCTATCCA	TAGAGCTGCGTGGTAACTCA	47.6	50.0	56.9	58.5	251-252 bp	1.0	63.7