Hotspot

atpH-atpF

LSC · 32284-32707 · 424 bp

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Pi 0.0463

MarkerSeek score 81.20

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 19
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TTATGGACTGGTTGTGGCAC	CGAATGGCCAGTGAGCTAAA	50.0	50.0	58.1	58.3	525-539 bp	1.0	82.1
atpH-atpF_p2	TGTGGCACTAGCGCTTTTAT	CGAATGGCCAGTGAGCTAAA	45.0	50.0	57.9	58.3	513-527 bp	1.0	82.1
atpH-atpF_p3	TTGTGGCACTAGCGCTTTTA	CGAATGGCCAGTGAGCTAAA	45.0	50.0	58.1	58.3	514-528 bp	1.0	82.1
atpH-atpF_p4	TTATGGACTGGTTGTGGCAC	ATTAAGCCCGAAACTCCCAG	50.0	50.0	58.1	57.6	545-559 bp	1.0	80.7
atpH-atpF_p5	TGTGGCACTAGCGCTTTTAT	ATTAAGCCCGAAACTCCCAG	45.0	50.0	57.9	57.6	533-547 bp	1.0	80.7