Hotspot

trnM-CAU-trnE-UUC

LSC · 14688-15211 · 524 bp

Back to Bouteloua

Pi 0.0462

MarkerSeek score 73.30

Primer available yes

Label trnM-CAU-trnE-UUC

Feature Metadata

label_name: trnM-CAU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 24
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnE-UUC_p1	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	601-606 bp	1.0	70.8
trnM-CAU-trnE-UUC_p2	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	580-585 bp	1.0	70.8
trnM-CAU-trnE-UUC_p3	TGGTAAGGCATAAGTCATCGG	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	606-611 bp	1.0	70.0
trnM-CAU-trnE-UUC_p4	GGTAAGGCATAAGTCATCGGT	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	605-610 bp	1.0	69.9
trnM-CAU-trnE-UUC_p5	TGGTAAGGCATAAGTCATCGG	TGTCCTGAACCACTAGACGA	47.6	50.0	57.5	57.7	585-590 bp	1.0	69.9