Hotspot

rps16-trnQ-UUG

LSC · 5549-6632 · 1084 bp

Back to Bouteloua

Pi 0.0331

MarkerSeek score 76.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1084
variable_sites: 35
pi: 0.0331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1149-1158 bp	1.0	82.5
rps16-trnQ-UUG_p2	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1160-1169 bp	1.0	80.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CGCATAAAACGAAATGGGGC	45.0	50.0	58.1	58.4	1211-1220 bp	1.0	79.3
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GCATAAAACGAAATGGGGCG	45.0	50.0	58.1	58.4	1210-1219 bp	1.0	79.3
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1153-1162 bp	1.0	77.8