Hotspot

ycf1

SSC · 125121-130778 · 5658 bp

Back to Bougainvillea

Pi 0.0119

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5658
variable_sites: 255
pi: 0.0119
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGTTCCGTTTCCATTCCCC	AAGACGAAGGTTTGGGATCG	50.0	50.0	58.1	57.9	845 bp	1.0	81.6
ycf1_p2	CATTCGATTCGTCCGTTGGA	AAGACGAAGGTTTGGGATCG	50.0	50.0	58.4	57.9	1083-1104 bp	1.0	79.8
ycf1_p3	TCATTCGATTCGTCCGTTGG	AAGACGAAGGTTTGGGATCG	50.0	50.0	58.4	57.9	1084-1105 bp	1.0	79.8
ycf1_p4	AAGTTCCGTTTCCATTCCCC	GTTTGGGATCGGAGGTTCAA	50.0	50.0	58.1	57.8	836 bp	0.933333	78.0
ycf1_p5	AAGTTCCGTTTCCATTCCCC	GATGCAATCATCGCCGATTC	50.0	50.0	58.1	57.8	975 bp	0.866667	75.2