Hotspot

rpl14-rpl16

LSC · 82898-83043 · 146 bp

Back to Bougainvillea

Pi 0.0186

MarkerSeek score 65.90

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 146
variable_sites: 11
pi: 0.0186
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	CGATCTCAATAGCGGCATCT	50.0	50.0	58.0	57.6	257-269 bp	1.0	84.1
rpl14-rpl16_p2	TCGACGATTGCTAGCTCCTA	CGATCTCAATAGCGGCATCT	50.0	50.0	58.0	57.6	287-299 bp	1.0	83.3
rpl14-rpl16_p3	ACACATCAATTCTCGAGCCC	CGAAGGGCGATCTCAATAGC	50.0	55.0	58.0	58.6	264-276 bp	1.0	82.2
rpl14-rpl16_p4	ACACATCAATTCTCGAGCCC	GAAGGGCGATCTCAATAGCG	50.0	55.0	58.0	58.6	263-275 bp	1.0	82.2
rpl14-rpl16_p5	TCGACGATTGCTAGCTCCTA	GAAGGGCGATCTCAATAGCG	50.0	55.0	58.0	58.6	293-305 bp	1.0	81.4