Hotspot

rpl20-rps12

LSC · 70715-71504 · 790 bp

Back to Bougainvillea

Pi 0.00681
MarkerSeek score 60.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
790
variable_sites
26
pi
0.00681
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 889-914 bp 1.0 83.9
rpl20-rps12_p2 TGAATGAGCCCCTCGAAAAG CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 887-912 bp 1.0 83.9
rpl20-rps12_p3 GAATGAGCCCCTCGAAAAGT CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 886-911 bp 1.0 83.9
rpl20-rps12_p4 TGAGCCCCTCGAAAAGTTTT CGCCGAGGAACATGTACTAG 45.0 55.0 57.6 57.9 883-908 bp 1.0 81.9
rpl20-rps12_p5 TGAATGAGCCCCTCGAAAAG GCCAATCAAAAACGTCACGA 50.0 45.0 57.9 57.6 936-961 bp 1.0 81.4