Hotspot

petA-psbJ

LSC · 64729-65732 · 1004 bp

Back to Bougainvillea

Pi 0.0145

MarkerSeek score 55.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1004
variable_sites: 39
pi: 0.0145
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCTCAGATGGACGTGAAGTG	GTCTCTAGCACGACCACTTG	55.0	55.0	58.0	58.0	1243-1500 bp	1.0	86.4
petA-psbJ_p2	CTCAGATGGACGTGAAGTGG	GTCTCTAGCACGACCACTTG	55.0	55.0	58.0	58.0	1242-1499 bp	1.0	86.4
petA-psbJ_p3	TCAGAGCTATCGCCCCAATA	GTCTCTAGCACGACCACTTG	50.0	55.0	58.0	58.0	1527-1784 bp	1.0	86.2
petA-psbJ_p4	CTCAGATGGACGTGAAGTGG	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1449-1706 bp	1.0	86.1
petA-psbJ_p5	CTCAGATGGACGTGAAGTGG	ACGACACAATCAAACCCGAA	55.0	45.0	58.0	58.0	1452-1709 bp	1.0	86.1