Hotspot

ndhC-trnV-UAC

LSC · 51869-52811 · 943 bp

Back to Bougainvillea

Pi 0.0102

MarkerSeek score 64.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 37
pi: 0.0102
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGTCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1036-1130 bp	1.0	84.9
ndhC-trnV-UAC_p2	GTCCTTTGCTAATCGGAGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1034-1128 bp	1.0	82.7
ndhC-trnV-UAC_p3	GGGTCCTTTGCTAATCGGAG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.0	57.5	1035-1129 bp	1.0	82.1
ndhC-trnV-UAC_p4	CTCGGGTCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1039-1133 bp	1.0	81.8
ndhC-trnV-UAC_p5	GGGTCCTTTGCTAATCGGAG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.0	58.7	1024-1118 bp	1.0	81.0