Hotspot

trnF-GAA-ndhJ

LSC · 49480-50162 · 683 bp

Back to Bougainvillea

Pi 0.00678

MarkerSeek score 61.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 20
pi: 0.00678
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGGTAGGATGG	50.0	55.0	58.1	58.0	781-794 bp	1.0	83.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGGTAGGATG	50.0	55.0	58.1	58.0	782-795 bp	1.0	83.8
trnF-GAA-ndhJ_p3	AAAGGGGAATGGTCGGGATA	CCCGAAAGTTGGGTAGGATG	50.0	55.0	58.1	58.0	832-845 bp	1.0	83.3
trnF-GAA-ndhJ_p4	AAAGGGGAATGGTCGGGATA	CCGAAAGTTGGGTAGGATGG	50.0	55.0	58.1	58.0	831-844 bp	1.0	83.3
trnF-GAA-ndhJ_p5	TGAGGAGATGATGCACCAGA	CCGAAAGTTGGGTAGGATGG	50.0	55.0	58.1	58.0	850-863 bp	1.0	83.0