Hotspot

trnT-UGU-trnL-UAA

LSC · 47672-48398 · 727 bp

Back to Bougainvillea

Pi 0.0121

MarkerSeek score 60.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 34
pi: 0.0121
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	538-803 bp	1.0	85.9
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	AGCCAATTAAGTCCGTAGCG	55.0	50.0	58.2	58.1	569-834 bp	1.0	85.6
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	526-791 bp	1.0	84.5
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	557-822 bp	1.0	84.1
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	55.0	50.0	57.9	58.4	537-802 bp	1.0	83.6