Hotspot

atpH-atpI

LSC · 14057-14783 · 727 bp

Back to Bougainvillea

Pi 0.00752

MarkerSeek score 65.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 20
pi: 0.00752
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	771-811 bp	1.0	83.4
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	787-827 bp	1.0	82.4
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	772-812 bp	1.0	82.2
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	831-871 bp	1.0	81.9
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	827-867 bp	0.933333	78.7