Hotspot

trnS-GCU-trnG-UCC

LSC · 8377-9376 · 1000 bp

Back to Bougainvillea

Pi 0.0233

MarkerSeek score 65.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 72
pi: 0.0233
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	55.0	38.1	58.6	55.5	874-1059 bp	1.0	56.4
trnS-GCU-trnG-UCC_p2	CTTTCGTCCACTCAGCCATC	AACGAATCACACTTTTACCACT	55.0	36.4	58.6	56.2	875-1060 bp	1.0	54.5
trnS-GCU-trnG-UCC_p3	TTTCGTCCACTCAGCCATCT	ACGAATCACACTTTTACCACT	50.0	38.1	59.0	55.5	873-1058 bp	1.0	53.3
trnS-GCU-trnG-UCC_p4	TCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	57.9	38.1	58.1	55.5	868-1053 bp	1.0	52.8
trnS-GCU-trnG-UCC_p5	CTTTCGTCCACTCAGCCATC	TACCACTAAACTATACCCGC	55.0	45.0	58.6	54.0	860-1045 bp	1.0	52.7