Hotspot

psbK-psbI

LSC · 7602-8012 · 411 bp

Back to Bougainvillea

Pi 0.0153

MarkerSeek score 58.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 23
pi: 0.0153
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGGATTACGCCCTGGATCAT	50.0	50.0	57.9	58.3	520-538 bp	1.0	83.9
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCGCGTCCAGGATTAC	50.0	50.0	57.9	57.7	532-550 bp	1.0	83.6
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	517-535 bp	1.0	81.9
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	518-536 bp	1.0	81.9
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	519-537 bp	1.0	81.9