Hotspot

trnK-UUU-rps16

LSC · 4453-5093 · 641 bp

Back to Bougainvillea

Pi 0.0183

MarkerSeek score 63.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 39
pi: 0.0183
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	ACGAATCCCTTGCTTCATCC	GGCGGGAGTTTTGGAAGAAT	50.0	50.0	58.0	58.5	645-792 bp	1.0	84.2
trnK-UUU-rps16_p2	CCGCACTTAAAAGCCGAGTA	GGCGGGAGTTTTGGAAGAAT	50.0	50.0	58.3	58.5	605-752 bp	1.0	82.5
trnK-UUU-rps16_p3	ACGAATCCCTTGCTTCATCC	ATTTTAAGGAGGGCGGGAGT	50.0	50.0	58.0	58.7	656-803 bp	1.0	82.2
trnK-UUU-rps16_p4	GAATCCCTTGCTTCATCCGA	GGCGGGAGTTTTGGAAGAAT	50.0	50.0	57.7	58.5	643-790 bp	1.0	81.9
trnK-UUU-rps16_p5	CATAGCCGCACTTAAAAGCC	GGCGGGAGTTTTGGAAGAAT	50.0	50.0	57.5	58.5	610-757 bp	1.0	81.0