Hotspot

trnH-GUG-psbA

LSC · 109-568 · 460 bp

Back to Bougainvillea

Pi 0.0212

MarkerSeek score 68.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 460
variable_sites: 40
pi: 0.0212
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	578-799 bp	1.0	85.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	592-813 bp	1.0	85.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	591-812 bp	1.0	85.0
trnH-GUG-psbA_p4	TGTTATGGACGTTATGGGCG	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.1	58.3	647-868 bp	0.866667	79.1
trnH-GUG-psbA_p5	TGTTATGGACGTTATGGGCG	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.1	57.6	648-869 bp	0.866667	78.7