Hotspot

rps15

SSC · 114378-114641 · 264 bp

Back to Bothriospermum

Pi 0.445

MarkerSeek score 76.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 110
pi: 0.445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ATGGTAAGGGTGCGGATAGA	GGTCTGTGCCCCAATATCTG	50.0	55.0	57.9	58.0	1298 bp	0.5	59.0
rps15_p2	ATGGTAAGGGTGCGGATAGA	CACCAATAATGCCTACGCCT	50.0	50.0	57.9	58.0	1542 bp	0.5	59.0
rps15_p3	ATGGTAAGGGTGCGGATAGA	TCCACCAATAATGCCTACGC	50.0	50.0	57.9	58.0	1544 bp	0.5	58.9
rps15_p4	ATGGTAAGGGTGCGGATAGA	TCCTAACTGTTCGGGTCCAT	50.0	50.0	57.9	58.1	1181 bp	0.5	58.8
rps15_p5	ATGGTAAGGGTGCGGATAGA	AACACCATGCATTGATGGGT	50.0	45.0	57.9	58.1	986 bp	0.5	58.8