Hotspot

psaC

SSC · 120315-120560 · 246 bp

Back to Bothriospermum

Pi 0.475

MarkerSeek score 76.30

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 106
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GGGCGATCTATTACCAACGG	GGGAGCCAGTGAATATTGGG	55.0	55.0	58.2	58.0	1148 bp	0.5	58.2
psaC_p2	GGGCGATCTATTACCAACGG	AAACCCGTGGTCGAAAATCA	55.0	45.0	58.2	58.0	1011 bp	0.5	58.1
psaC_p3	AGAGCCCTTATGTGTCTGGA	GGGAGCCAGTGAATATTGGG	50.0	55.0	57.7	58.0	944 bp	0.5	57.9
psaC_p4	AGAGCCCTTATGTGTCTGGA	AAACCCGTGGTCGAAAATCA	50.0	45.0	57.7	58.0	807 bp	0.5	57.7
psaC_p5	GGGCGATCTATTACCAACGG	TTTGACCGCTGTACATTGCT	55.0	45.0	58.2	58.1	1466 bp	0.5	57.5