Hotspot

ndhI

SSC · 118163-118663 · 501 bp

Back to Bothriospermum

Pi 0.52

MarkerSeek score 79.70

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 230
pi: 0.52
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GCGATGGAGCAGTGATGTTA	CCCACGGTCCAAAGATGAAA	50.0	50.0	58.1	58.1	1770 bp	0.5	58.8
ndhI_p2	GCGATGGAGCAGTGATGTTA	GCGGCAGCTACAAAATAGGA	50.0	50.0	58.1	58.3	1665 bp	0.5	56.8
ndhI_p3	GCGATGGAGCAGTGATGTTA	CGTAAATAAGGAGTTGCGCG	50.0	50.0	58.1	57.7	1682 bp	0.5	56.7
ndhI_p4	GCGATGGAGCAGTGATGTTA	TCCCGTACCATGACGTATCT	50.0	50.0	58.1	57.6	1850 bp	0.5	56.7
ndhI_p5	GCGATGGAGCAGTGATGTTA	CCCGATCCCAGAGAGACTAA	50.0	55.0	58.1	57.6	1536 bp	0.5	56.6