Hotspot

ndhH

SSC · 114742-115923 · 1182 bp

Back to Bothriospermum

Pi 0.461

MarkerSeek score 81.50

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 476
pi: 0.461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TTGGGAAAACGTCAACGACT	TAGGGCTTGAAGGATTCCCA	45.0	50.0	58.0	58.0	1616 bp	0.5	59.6
ndhH_p2	TTGGGAAAACGTCAACGACT	GCTAGGGCTTGAAGGATTCC	45.0	55.0	58.0	58.0	1618 bp	0.5	59.6
ndhH_p3	TTGGGAAAACGTCAACGACT	AGTCCAAGGGGAGCAATACT	45.0	50.0	58.0	58.0	1822 bp	0.5	59.6
ndhH_p4	TTGGGAAAACGTCAACGACT	CATCTGCTAGGGCTTGAAGG	45.0	55.0	58.0	58.0	1623 bp	0.5	59.5
ndhH_p5	TTGGGAAAACGTCAACGACT	CCATCTGCTAGGGCTTGAAG	45.0	55.0	58.0	58.0	1624 bp	0.5	59.5