Hotspot

ndhG

SSC · 118998-119528 · 531 bp

Back to Bothriospermum

Pi 0.458

MarkerSeek score 79.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 207
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TCAAATTGCGTTAGGTCGGT	CCGTTGGTAATAGATCGCCC	45.0	55.0	57.8	58.2	1083 bp	0.5	57.0
ndhG_p2	GAGGCCGAATCCACTTTGAA	CCGTTGGTAATAGATCGCCC	50.0	55.0	58.2	58.2	1336 bp	0.5	56.9
ndhG_p3	TCAAATTGCGTTAGGTCGGT	TCCAGACACATAAGGGCTCT	45.0	50.0	57.8	57.7	1287 bp	0.5	56.6
ndhG_p4	GAGGCCGAATCCACTTTGAA	TCCAGACACATAAGGGCTCT	50.0	50.0	58.2	57.7	1540 bp	0.5	56.5
ndhG_p5	TCAAATTGCGTTAGGTCGGT	CCAGTCCAATAGCTGCTTCA	45.0	50.0	57.8	57.6	1418 bp	0.5	55.4