Hotspot

ndhF

SSC · 124918-127128 · 2211 bp

Back to Bothriospermum

Pi 0.45

MarkerSeek score 80.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2211
variable_sites: 894
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCTTTCAAAGATCCGGCGAA	GCAGAATACCGTCACCCATT	45.0	50.0	57.5	58.0	2916 bp	0.5	56.0
ndhF_p2	TTCTTTCAAAGATCCGGCGA	GCAGAATACCGTCACCCATT	45.0	50.0	57.5	58.0	2917 bp	0.5	56.0
ndhF_p3	TCTTTCAAAGATCCGGCGAA	ACAGGAACAAGAGGGATCCA	45.0	50.0	57.5	58.0	2750 bp	0.5	55.9
ndhF_p4	TTCTTTCAAAGATCCGGCGA	ACAGGAACAAGAGGGATCCA	45.0	50.0	57.5	58.0	2751 bp	0.5	55.9
ndhF_p5	TCTTTCAAAGATCCGGCGAA	ACCGTCACCCATTCTGACTA	45.0	50.0	57.5	58.1	2909 bp	0.5	55.7