Hotspot

ndhD

SSC · 120681-122231 · 1551 bp

Back to Bothriospermum

Pi 0.474

MarkerSeek score 80.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1551
variable_sites: 613
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTGCTCCAAGAACAGAGGA	GGGCTACACAGTTACGGTTT	50.0	50.0	58.0	57.8	2009 bp	0.5	58.5
ndhD_p2	TGATTTTCGACCACGGGTTT	GGGCTACACAGTTACGGTTT	45.0	50.0	58.0	57.8	1814 bp	0.5	58.4
ndhD_p3	TCTGCTCCAAGAACAGAGGA	GCAATTGTGGCGTCAATAGG	50.0	50.0	58.0	57.8	2078 bp	0.5	58.3
ndhD_p4	TCTGCTCCAAGAACAGAGGA	AACTGTGGGGATTCTTTCGG	50.0	50.0	58.0	57.8	2235 bp	0.5	58.3
ndhD_p5	TCTGCTCCAAGAACAGAGGA	TGCAAATTCCGCAATTGTGG	50.0	45.0	58.0	58.2	2088 bp	0.5	58.2