Hotspot

ndhG-ndhE

SSC · 119529-119758 · 230 bp

Back to Bothriospermum

Pi 0.518

MarkerSeek score 75.10

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 230
variable_sites: 116
pi: 0.518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	TTTCATCTTTGGACCGTGGG	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.1	58.0	1190 bp	0.5	59.1
ndhG-ndhE_p2	TTTCATCTTTGGACCGTGGG	CAGTCCTCTGTTCTTGGAGC	50.0	55.0	58.1	57.9	1193 bp	0.5	58.5
ndhG-ndhE_p3	TTTCATCTTTGGACCGTGGG	GTCCTCTGTTCTTGGAGCAG	50.0	55.0	58.1	57.9	1191 bp	0.5	58.5
ndhG-ndhE_p4	TTTCATCTTTGGACCGTGGG	TGCTTTACATCCGTCCCAAG	50.0	50.0	58.1	57.9	1158 bp	0.5	58.4
ndhG-ndhE_p5	TTTCATCTTTGGACCGTGGG	CTGTTCTTGGAGCAGAAGCA	50.0	50.0	58.1	58.1	1186 bp	0.5	58.3