Hotspot

trnN-GUU-rps15

SSC · 108306-114377 · 6072 bp

Back to Bothriospermum

Pi 0.299

MarkerSeek score 73.00

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: SSC
length_bp: 6072
variable_sites: 1442
pi: 0.299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	AAAAGCTAAGCGTGTGGTCA	AAATGAGGGGCGCTTTCTAG	45.0	50.0	58.0	58.0	805 bp	0.5	59.3
trnN-GUU-rps15_p2	AAAAGCTAAGCGTGTGGTCA	ACCAAGCCGGAGATTGAATC	45.0	50.0	58.0	58.0	1085 bp	0.5	59.3
trnN-GUU-rps15_p3	AAAAGCTAAGCGTGTGGTCA	GACCAAGCCGGAGATTGAAT	45.0	50.0	58.0	58.0	1086 bp	0.5	59.3
trnN-GUU-rps15_p4	TTCTTGAACGGGCCATATCG	AAATGAGGGGCGCTTTCTAG	50.0	50.0	58.1	58.0	1116 bp	0.5	59.2
trnN-GUU-rps15_p5	TTCTTGAACGGGCCATATCG	ACCAAGCCGGAGATTGAATC	50.0	50.0	58.1	58.0	1396 bp	0.5	59.2