Hotspot

ndhF-rpl32

SSC · 104506-105379 · 874 bp

Back to Bothriochloa

Pi 0.00701

MarkerSeek score 61.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 874
variable_sites: 9
pi: 0.00701
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	940-959 bp	1.0	43.8
ndhF-rpl32_p2	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	941-960 bp	1.0	43.8
ndhF-rpl32_p3	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	942-961 bp	1.0	43.8
ndhF-rpl32_p4	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	943-962 bp	1.0	43.8
ndhF-rpl32_p5	CTGGAAGTGGGAGAAGAGGA	TTTTTGACATCGAAGTACGT	55.0	35.0	58.1	53.2	944-963 bp	1.0	43.8