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psbM-petN

LSC · 16171-16774 · 604 bp

Back to Bothriochloa

Pi 0.00331

MarkerSeek score 59.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 3
pi: 0.00331
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGTTCATTCTAGTTCCTACTGCC	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.9	58.2	750-755 bp	1.0	55.1
psbM-petN_p2	TGTTCATTCTAGTTCCTACTGCC	GTAGTATGGGGGAGGAGTGG	43.5	60.0	57.9	58.3	694-699 bp	1.0	54.1
psbM-petN_p3	TGTTCATTCTAGTTCCTACTGCC	TAGTATGGGGGAGGAGTGGA	43.5	55.0	57.9	58.4	693-698 bp	1.0	53.4
psbM-petN_p4	GTTCATTCTAGTTCCTACTGCCT	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.7	58.2	749-754 bp	1.0	53.0
psbM-petN_p5	TGTTCATTCTAGTTCCTACTGCC	CGCTAGTAGTATGGGGGAGG	43.5	60.0	57.9	58.5	699-704 bp	1.0	52.7