Hotspot

trnD-GUC-psbM

LSC · 15008-16065 · 1058 bp

Back to Bothriochloa

Pi 0.00441

MarkerSeek score 59.20

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 7
pi: 0.00441
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.7	57.9	1214-1219 bp	1.0	53.6
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	1180-1185 bp	1.0	52.1
trnD-GUC-psbM_p3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	57.7	57.7	1215-1220 bp	1.0	51.5
trnD-GUC-psbM_p4	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	57.7	55.5	1215-1220 bp	1.0	50.2
trnD-GUC-psbM_p5	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.7	54.5	1175-1180 bp	1.0	50.1