Hotspot

ycf1

SSC · 128245-133371 · 5127 bp

Back to Boswellia

Pi 0.00934

MarkerSeek score 46.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5127
variable_sites: 152
pi: 0.00934
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCGGGCGATATTTTTAGCT	ACTCCCAACGCTCAAACAAT	45.0	45.0	58.0	58.0	667-769 bp	1.0	89.8
ycf1_p2	ACCACTCGGCAACTTGAAAT	ACTCCCAACGCTCAAACAAT	45.0	45.0	58.0	58.0	694-796 bp	1.0	89.5
ycf1_p3	CGTTTTAGGGGTGGACCAAA	ACTCCCAACGCTCAAACAAT	50.0	45.0	58.0	58.0	959-1061 bp	1.0	86.9
ycf1_p4	CGTTTTAGGGGTGGACCAAA	TTCAACTCCCAACGCTCAAA	50.0	45.0	58.0	58.0	963-1065 bp	1.0	86.7
ycf1_p5	CGTTTTAGGGGTGGACCAAA	TTTCAACTCCCAACGCTCAA	50.0	45.0	58.0	58.0	964-1066 bp	1.0	86.7