Hotspot

ndhF

SSC · 113666-115900 · 2235 bp

Back to Boswellia

Pi 0.00482

MarkerSeek score 40.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 32
pi: 0.00482
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCGATT	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.1	57.9	2560-2607 bp	1.0	80.6
ndhF_p2	CCTCGGAAAGGCAAGAAAGT	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.1	57.9	2518-2565 bp	1.0	80.3
ndhF_p3	GCAGAATACCGTCACCGATT	GAGCCGGTGAATCAGAAACA	50.0	50.0	58.1	58.2	2559-2606 bp	1.0	79.8
ndhF_p4	AGGGGGACTAAACAGGAACA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	57.9	57.9	2464-2512 bp	0.857143	73.6
ndhF_p5	GTCGAAAGAGAGGGGGACTA	AGAGCCGGTGAATCAGAAAC	55.0	50.0	57.9	57.9	2474 bp	0.714286	67.0